Phytochemical screening, physicochemical parameters and in-vitro anti-inflammatory activity of mussaenda macrophylla
Pdiangmon Kyndait, Kirangngaulule Elu, TC Lalhriatpuii
To evaluate the phytochemical screening, physicochemical parameters and in-vitro anti-inflammatory activity of Mussaenda macrophylla. The fresh plant of Mussaenda macrophylla was collected and were shade dried and extracted with petroleum ether, chloroform and methanol by maceration method. Preliminary phytochemical screening of the extract was done for the identification of pharmacologically actives substances found in the plant. Estimation of proximate composition of the plant and moisture content, Ash value content and fluorescence analysis of the plant powder were also determined. The in-vitro anti-inflammatory activity of the methanolic extract was evaluated by HRBC membrane stabilization method. The phytochemical screening of the extracts of Mussaenda macrophylla was found to contain reducing sugar, phenolic, flavonoids and, steroids. The percentage of moisture content in the plant is 7.72%, total ash is 6.00%, water-soluble ash is 1.33% and acid insoluble ash is 3.66%. In fluorescence analysis different colors were observed which indicated the presence of phytochemical constituents. In in-vitro anti-inflammatory activity the IC50 value of methanolic extract was found to be 44.57%. The phytochemical constituents of the extracts were well known pharmacologically active chemicals and significant anti-inflammatory potential was shown by the methanolic extract. Thus, Mussaenda macrophylla can be considered as good source of biological activity.