Vol. 4, Issue 4 (2019)
The analysis of giving kebar grass extract (Biophytum Petersianum) toward malondialdehyde levels and spermatozoa morphology balb/c male mice that exposed By cigarrette smoke
Author(s): Dwi Ulfa Ningsih, Melyana Nurul Widyawati, Budiyono
Abstract: Background: Exposure to cigarette smoke increases reactive oxygen species, decreases natural antioxidants resulting in damage to cells, tissues and inhibits the process of spermatogenesis. Kebar grass extract contains antioxidants, protecting cells and tissues from damage caused by exposure to free radicals. Objective: To determine effect of giving different doses of kebar grass extract to the Malondialdehyde levels (MDA) and spermatozoa morphology Balb/C male mice that exposed by cigarette smoke. Method: The research using a post test only control group design. Total sample of 18 Balb/C male mice divided into 3 groups. All groups were exposed to non-filter cigarette smoke with tar levels of 39 mg and nicotine 2.3 mg. The control group (K) was only given exposure to cigarette smoke. Experiment Group1 (E1) was exposed to cigarette smoke and kebar grass extract at a dose of 0.75 gr / 25 mL aquades. Experiment Group2 (E2) was exposed to cigarette smoke and kebar grass extract at a dose of 1.25 gr / 25 mL aquades. The treatment was given for 28 days, on the 29th day all mice were terminated and Malondialdehyde levels (MDA) and spermatozoa morphology were examined. Results: The results test One Way Anova and Post hoc Multiple Comparisons showed p value = 0.001, so it can be concluded that kebar grass extract influences Malondialdehyde levels (MDA) and spermatozoa morphology of Balb/C male mice that exposed to cigarette smoke with an average MDA level of K group = 7.86 µL / ml; E1= 5.58 µL / ml; E2= 3.26 µL / ml and morphology group K = 80.83%; E1= 88.5%; E2= 93.66%. Conclusion: The use a dose of 0.75 gr and 1.25 gr / 25 mL of distilled water for MDA levels has the same effect, but a dose of 1.25 gr / 25 mL of distilled water for morphology has the best results.