Vol. 4, Issue 1 (2019)
Study of bacteriological profile and antibiotic susceptibility pattern in patients with neonatal sepsis at a tertiary care teaching hospital in Solapur, Maharashtra
Author(s): Dr. Anu Sharma, Dr. Shaikh Najmeen Abdul Rauf, Dr. PH Waghmare
Abstract: Introduction: Neonatal septicemia refers to bacterial infection in first four week of life which can be confirmed by growth on blood culture. It is responsible for high rates of Neonatal mortality. Aim of the study is to know the bacteriological profile and antibiotic sensitivity pattern among neonates clinically suspected for sepsis. Material & Method: Blood sample received for culture and sensitivity from NICU were incubated for 14 days and subcultures were done every alternate day. In case of growth, organisms were identified using standard protocols and antibiotic susceptibility testing was done by Kirby bauer disc diffusion method as per CLSI guidelines. Results: Total samples received between August 2017 to September 2018 from NICU were 303 out of which 102 (33.66%) were culture positive. Among 102 samples majority of isolates were gram negative bacteria (n=52) out of which Acinetobacter baumannii 20 (38.46%) followed by Klebsiella pneumoniae 18 (34.61%) were most common. Among Gram positive bacteria Staphylococcus aureus (n=44) 43% were most commonly isolated, out of which MRCONS were 30 (38%), MRSA 12 (27%) and MSSA were 02 (4.54%), Enterococcus faecalis 06 (5.8%) were also isolated. Among Gram positive bacteria maximum sensitivity was seen for vancomycin 100% followed by Gentamicin 31%, Piperacillin tazobactam 33.3% Linezolid 33.3%. Among Gram negative bacteria maximum sensitivity was seen for colistin 100% followed by piperacillin tazobactam 42%, Impienem 30%, Amikacin and Ciprofloxacin 28% respectively. Maximum resistance was seen for ampicillin 77% and cefotaxime 75%.