The purpose of the study was “Comparison of self-concept in male and female kho-kho players”. The subject for this study was 12th South Asian Games 2016, which was organized by India in Guwahati (Assam). Total 90 (ninety) male and female Kho-Kho players, 45 male [15 players of India,15 players of Nepal,15 players of Bangladesh] and 45 female [15 players ofIndia,15 players of Nepal,15 players of Bangladesh players] were (19-30 years of age) selected. Self-concept questionnaire developed by Robson  were selected for this. The selected variables was self-concept. The obtained data were analyzed by applying independent‘t’ test in order to comparison of self-concept differential between male and female Kho-Kho players. Further, the level of significance was set at 0.05 level of confidence. The data obtained after scoring the questionnaires is to be statistically analyzed on computer through Statistical Package for Social Science (SPSS) version 15.0.The finding of the study reveals that there was no significant difference in self-concept. The insignificant difference was found in case of self-concept between male and female kho-kho players may be due to the reason that the kho-kho players were highly motivated to win 12th South Asian Games, they undergone with same training, tough schedule and equal level of experience and a very high level of fitness which must have been a probable cause.